No abstract available.
Ganglia ; Hemolytic-Uremic Syndrome

No abstract available.
Ganglia ; Hemolytic-Uremic Syndrome

No abstract available.
Albuterol* ; Asthma* ; Gases* ; Inhalation*

In order to determine the enzyme activity, as expressed in Reitman-Frankel unit, of GOT isozyme present in whole homogenate, mitochondrial fraction and supernatant fraction were prepared from brain tissues of normal adult rabbit, by a differential centrifugal method. The effect of thiopental on the GOT isozyme activity in each fraction was determined and the following results were obtained. 1) The activity of GOT isozyme in whole homogenate of normal rabbit brain tissues was found to be 545+/-2.608 units/mg of wet weight whereas the corresponding figure for the supernatant GOT isozyme was 512+/-3.081 and the value for the mitochondrial GOT isozyme was found to be 34.9+/-1.224. 2) The supernatant GOT isozyme existing in a floating status within the cytoplasm accounted for 94 percent followed by 6.35 percent of mitochondrial GOT isozyme. 3) The activated-peak of mitochondrial GOT isozyme contained in the whole homogenate of adult rabbit brain tissues was found to be at #15 on the tube of elution in comparison to that of #73 for supernatant GOT isozyme, as analyzed by the DEAE-Cellulose column chromasography. 4) The supernant GOT isozyme from the thiopentaltreated brain was proportionaly distorted while mitochondrial GOT isozyme was not influenced. Fro example, treated with thiopental, the supernatant GOT isozyme was divided to be #63 & #73 on the tube in comparison to #15 for the mitochondrial GOT isozyme. 5) The activity of supernatant isozyme was proportionaly reduced as the concentration of thiopental. 6) Fifty percent inhibition dose(1se) of thiopental on the supernatant GOT isozyme was found to be 0.63mM. 7) The inhibitory effect of thiopental on the supernatant GOT isozyme was very high significantly by the statistics. 8) The mchanism by which thiopental inhibits the supernatant GOT isozyme in the adult rabbit brain was found to bh uncompetitive inhibition as its Michaelis-Menten constant Km=58.07mM demonstrated. In view of the above finding it is suggested that the thiopental inhibited selectively the activity of supernatant GOT isozyme of the adult rabbit brain tissues while it did not inhibitnificantly by the statistics. 8) The mechanism by which thiopental inhibits the supernatant GOT isozyme in the adult rabbit brain was found to bh uncompetitive inhibition as it Michaelis-Menten constant of Km=58.07 mM demonstrated. In view of the above findings it is suggested that the thiopental inhibited selectively the activity of supernatant GOT isozyme of the adult rabbit brain tissues while it did not inhibit that of mitochondrial GOT isozyme. The GOT isozyme of adult rabbit brain tissues was divided into thiopental-sensitive GOT isozyme(supernatant GOT isozyme) and thiopental insensitive GOT isozyme(mitochondrial GOT isozyme) Furthermore, it is suggested that the cellular function of the brain can be somewhat hindered, when thiopental is injected into the brain cell, while mitosis of the brain cell is not influenced.
Adult* ; Aspartate Aminotransferases* ; Brain* ; Cytoplasm ; DEAE-Cellulose ; Humans ; Mitosis ; Thiopental*

No abstract available.
Gastroschisis

No abstract available.
Gastroschisis

Biliary cystadenomas and cystadenocarcinomas are rare tumors of biliary origin and constitute less than 5% of the total intrahepatic cysts of bile duct origin. They occur most commonly in middle age females. Clinical manifestations are non-specific, so these tumors are incidentally found during autopsy or surgery. The ultrasonogram, abdominal CT scan, angiography and aspiration cytology are valuable diagnostic methods. The typical sonographic appearance of these tumors is a large intrahepatic globular or ovoid thick-walled cystic mass which often contains multiple septations or papillary infoldings. The characteristic CT appearance of these tumors is similar to that of a low-density intrahepatic mass which may cantain mural nodule or internal septation. However, regardless of the diagnostic modalities, pre-operative diagnosis as well as intra-operative one is difficult. Since biliary cystadenocarcinomas are believed to arise from benign cystadenornas, complete surgical resections, if possible, is a warranted method to avoid malignant change of these tumor. We report one of biliary cystadenocarcinoma canfirmed by the operation with review of the literatures.
Angiography ; Autopsy ; Bile Ducts ; Cystadenocarcinoma* ; Cystadenoma ; Diagnosis ; Female ; Humans ; Middle Aged ; Tomography, X-Ray Computed ; Ultrasonography

Hepatic cysts are usually asymptomatic and require no treatment. Their presence is detected incidentally by ultrasound or computed tomography, but occasionally some of them become large enough to cause symptom. Aspiration of cystic fluid alone, surgical intervention or instillation of various sclerosing agents has been performed. There have been few case reports of hepatic cysts treated successfully by aspiration and injection of minocycline chloride. We experienced a successful treatment of a huge (24 cm in diameter) hepatic cyst by aspiration and one-shot instillation of minocycline chloride. After cystie fluid was aspirated with pig tail catheter, minocycline chloride (500 mg in 10 ml of 1% Lidocaine) was instillated into the hepatic cyst. Six months after the treatment, fluid did not reaccumulate and regressed mostly. Here, we report a case of successful sclerotherapy of huge symptomatic hepatic cyst with one shot instillation of minocycline chloride,
Catheters ; Minocycline* ; Sclerosing Solutions ; Sclerotherapy ; Ultrasonography

No abstract available.
Female ; Ovary* ; Teratoma*

The aim of the present study was to investigate the mRNA expression of several cytokines which were not reported previously from interface tissues around loose cemented acetabulum to obtain better understanding of the biological mechanisms connected with aseptic loosening and osteolysis of THA. We investigated mRNA expression for several cytokines (interleukin-1 alpha [IL-l~a], IL-lp, IL-2, IL-2 receptor[2R], IL-4, IL-5, IL-8, IL-10, transforming growth factor-beta [TGF-p], and interferon- gamma [IFN-y]) by reverse transcription-polymerase chain reaction (RT-PCR) and release of metalloproteinase (MMP)-2 and MMP-9 from the cement-bone interface tissues around five loose polyethylene acetabular components. We did not include TNF-a and IL-6 because the biologic effect of the former is so similar to that of IL-1, and the latter fails to stimulate prostaglandin E, or collagenase production by fibroblsts or synovial cells. Expression of mRNA for IL-1p was detected in four, IL-2R and IL-8 in three, IL-10 and TGF-p in two of five interface tissues .No expression of mRNA for IL-la, IL-2, IL-4, IL-5, and IFN-p was detected. Zymographic analysis for gelatinase/type IV collagenase revealed gelatinolytic bands corresponding to metalloproteinase(MMP)-2 and MMP-9 in cemenl-bone interface tissues. Activated cells phagocytose particles in cement-bone interface tissues expressed more cytokines mRNA than previously known to be related to periprosthetic bone resorption, and secreted metalloproteinases associated with extracellular matrix degradation and fibrosis.
Acetabulum ; Arthroplasty, Replacement, Hip* ; Bone Resorption ; Collagenases ; Cytokines* ; Extracellular Matrix ; Fibrosis ; Interleukin-1 ; Interleukin-10 ; Interleukin-2 ; Interleukin-4 ; Interleukin-5 ; Interleukin-6 ; Interleukin-8 ; Metalloproteases* ; Osteolysis ; Polyethylene ; RNA, Messenger*