BACKGROUND: The influencion the environment on a culture is expressed via four routes. (1) the nature of the substrate or phase on or in which the cells grow (2) the physicochemical and physiological constitution of the medium, (3) the constitution of the gas phase, and (4) the incubation temperature. Melanization is closely related to the constitution and amounts of amino acids in the medium. OBJECTIVE: The aim of this study is to investigate whether there are some differences of proliferation and melanization in cultured B,F, mouse melanoma cells according to different culture media. METHODS: We examined the color of cell pellet, cell morphology, electron microscopic findings, cell counts and melanin conlensin BgF mouse melanoma cells cultured in Dulbeccos modified Eagles medium(DMEM), F-10, MCDB 153, Minimal essential medium(MEM), and RPMI 1640, respectively. RESULTS: 1. The color of cell pellet., ringed from dark gray to light brown. The order of the darkness was DMEM, MEM, RPMI 1640, MCDB 153, and F-10 medium. 2. Most Bg, mouse melanoma cells had an epithelioid morphology, but a few cells in MCDB 153 medium showed dendrites. On the 4th day after culture, the cells in F-10 medium were larger than those in the other media. 3. In the electron microscopic. findings, BF, mouse melanoma cells in DMEM and MEM con tained numerous stage IV nelanosomes, however, those in RPMI 1640 and MCDB 153 medium contained a few, and those in F-10 medium did few. 4. The number of BF, mouse melanoma cells were 1.42 + 0.06 x 10", 1.42 + 0.12 x 10", l. 17 + 0.08 x 10, 0.73 0.06 x 10, 0.32 0.01 x 10, in RPMI 1640, DMEM, MEM, F 10, and MCDB 153 medium, respectively. 5. In the MTT assay, the order of the optical density of B,F, mouse melanoma cells in various media was as followings, DMEM, RPMI 1640, MEM, F-10, and MCDB 153. 6. Compared with the melanin contents of B;F, mouse melanoma cells in DMEM, they were 77.97% in MEM, 67.91% in RPMI 1640 and MCDB 153 medium, and 55.94% in F-10 medium. CONCLUSION: The phenotypic changes of BF, mouse melanoma cells were induced by various culture rnedia and were reversilvle. Since the phenotypes of cells can be changed by the culture media, researchers should choose the appropriate culture medium for the cells.
Amino Acids ; Animals ; Cell Count ; Constitution and Bylaws ; Culture Media ; Darkness ; Dendrites ; Eagles ; Melanins ; Melanoma* ; Mice* ; Phenotype

No abstract available.

In 1968, Hardy and Anderson first described the term hypereosinophilic syndrome. This syndrome is characterized by persistent and prolonged eosinothila, with primarily hematologic, cardiac, neurologic and derrnatologic abnormalities. Cutaneous mmestations occur in 27-57% of patients. Two types of skin lesions have been noted: (1) erythrritous pruritic papules and nodules or (2) urticaria and angioedema. We report a case of hypereosinophilic syndrome with a vesicle eruption which is a rare skin lesion in tbis syndrome, in a 17 year old man. Diagnosis of hyprcsinophilic syndrome was established by clinical findings, rearked blood eosinophilia without a Brown cause, bone marrow aspiration and biopsy, liver scan and ultrasonography, and histopatholcgie findings of the skin.
Adolescent ; Angioedema ; Biopsy ; Bone Marrow ; Diagnosis ; Eosinophilia ; Humans ; Hypereosinophilic Syndrome* ; Liver ; Skin ; Ultrasonography ; Urticaria

No abstract available.
Immunosuppression* ; Prognosis* ; Transplantation*

No abstract available.
Bile* ; Drainage*

OBJECTIVE: The purpose of this study was to develop the decision tree models to classify the characteristics of those who had not undergone the health screening tests provided by the National Health Insurance Corporation. METHODS: Total of 5,102,761 subjects of health screening services in the year of 2002 was used. The data was divided into two data-sets (disease VS. non-disease group). The target variable was whether they took the health screening services. The number of input variables was 25 in total. RESULTS: The decision trees were classified into fourteen different types of non-examinees in the non-disease group and nineteen in the disease group. The ROC curve areas in the non-disease and disease groups were .761 and .714, respectively. CONCLUSION: The different types of non-examinees classified by the decision tree models would facilitate the foundation for the further analysis of individual characteristics and the effective health screening service management in future.
Data Mining ; Decision Trees* ; Mass Screening* ; National Health Programs ; ROC Curve

Using a monoclonal antibody OKT 6, we nvestigated Langerhans cells by indirect immunoperoxidase technique in 60 skin specimens of fetuses from 3 months of gestation period to 10 months and 21 skin specimens of adults for the study of their development and the comparison of their density in fetus with adult. The results were as follows: 1. Langerhans cell population density of fetus was 52/mm in 4 months of gestation period, l07/mm in 5 months,136/mm in 6 months, l97/mm in 7 months, 235/mm in 8 months, and 311/mm in 10 months and there was a tendency to increase from 4 months to 1Q months. The earliest fetal skin specimen showing OKTg(+) Langerhans cell was that of 13 weeks and 2 days of gestation period. 3. Langerhans cell population density of back skin in fetus was 12.6% in 5 months, 16.0%, in 6 months, 20.8% in 7 months, 26.4% in 8 months and 36, 2% in 10 months of 900/mm in adult back skin. 4, Langerhans cell population density in adult was 432/mm in the skin of shin, 363/mm in prepuce, and 90Q/mm in back skin and there was statistically significant in difference between prepuce and back skin(p<0.00l).
Adult ; Fetus* ; Humans ; Immunoenzyme Techniques ; Langerhans Cells* ; Population Density ; Pregnancy ; Skin

Using a monoclonal antibody OKT 6, we nvestigated Langerhans cells by indirect immunoperoxidase technique in 60 skin specimens of fetuses from 3 months of gestation period to 10 months and 21 skin specimens of adults for the study of their development and the comparison of their density in fetus with adult. The results were as follows: 1. Langerhans cell population density of fetus was 52/mm in 4 months of gestation period, l07/mm in 5 months,136/mm in 6 months, l97/mm in 7 months, 235/mm in 8 months, and 311/mm in 10 months and there was a tendency to increase from 4 months to 1Q months. The earliest fetal skin specimen showing OKTg(+) Langerhans cell was that of 13 weeks and 2 days of gestation period. 3. Langerhans cell population density of back skin in fetus was 12.6% in 5 months, 16.0%, in 6 months, 20.8% in 7 months, 26.4% in 8 months and 36, 2% in 10 months of 900/mm in adult back skin. 4, Langerhans cell population density in adult was 432/mm in the skin of shin, 363/mm in prepuce, and 90Q/mm in back skin and there was statistically significant in difference between prepuce and back skin(p<0.00l).
Adult ; Fetus* ; Humans ; Immunoenzyme Techniques ; Langerhans Cells* ; Population Density ; Pregnancy ; Skin

No abstract available.
Cholangiopancreatography, Endoscopic Retrograde* ; Pancreas* ; Pancrelipase*

Boric acid(H BO) is a weak topical anti infective agent. It can cause acute borism due to the internal absorption. Although the frequency is very rare, boric acid can also induce contact dermat.itis after topical spplication. We present allergic contact dermatitis to boric acid. The patch test results showed a positive reaction to 2% boric acid solution.
Absorption ; Dermatitis, Allergic Contact* ; Patch Tests