The role of parvalbumin interneuron-mediated disinhibition in the antidepressant effects of ketamine
10.16571/j.cnki.1008-8199.2020.07.003
- VernacularTitle: 微清蛋白中间神经元介导的去抑制在氯胺酮抗抑郁中的作用
- Author:
Feng ZHOU
1
;
Xiao-hui TANG
2
;
Ru LIU
1
;
Jian-jun YANG
3
;
Zhi-qiang ZHOU
1
;
Qing JI
1
Author Information
1. Department of Anesthesiology, Jinling Hospital, Nanjing University School of Medicine/General Hospital of Eastern Theater Command, PLA, Nanjing 210002, Jiangsu, China
2. Department of Anesthesiology, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009,Jiangsu,China
3. Department of Anesthesiology, Pain and Perioperative Medicine,the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000,Henan,China
- Publication Type:Journal Article
- Keywords:
Ketamine;
Antidepressant;
Parvalbumin;
Hippocampus
- From:
Journal of Medical Postgraduates
2020;33(7):684-688
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the role of parvalbumin interneuron-mediated disinhibition in the antidepressant effects of ketamine.MethodsForty-eight adult male C57BL/6 mice were randomly divided into the following four groups (n=12): group Saline + Saline (group SS), group LPS + Saline (group LS), group Saline + Ketamine (group SK) and group LPS + Ketamine (group LK). The depression model was established by intraperitoneal injection of LPS (1 mg/kg), and ketamine (10 mg/kg) or physiological saline (equal volume) intraperitoneally injected 20 hours later. Four hours after ketamine administration, the open field test and the forced swimming test were performed. The hippocampus was harvested after the behavioral test. PCR and Western blot were used to detect the expressions of PV and GAD67. Electrophysiology were used to detect the change of miniExcitatory post-synaptic current of pyramidal neurons in hippocampus CA1 region.ResultsCompared with the group SS, the time spent in the center zone of the arena was significantly decreased, the immobility time was significantly increased, the mRNA and protein content of PV were significantly increased, the amplitude and frequency of miniEPSC were significantly decreased in the group LS (P<0.05). Compared with the group LS, the time spent in the center zone of the arena was significantly increased, the immobility time was significantly decreased, the mRNA and protein content of PV were significantly decreased, the amplitude and frequency of miniEPSC were significantly increased in the group LK (P<0.05). In the open field test, no significantly difference was observed in the total traveled distance among the four groups (P>0.05).ConclusionKetamine can exert rapid antidepressant effects by down-regulating the expression of PV and then exerting disinhibition regulation on pyramidal neurons.
